Protocols
Protocols previously used or currently in use in the lab.
Enzymatic Activity Assays
Several spectrophotometric methods to measure the activities of antioxidant and metabolic enzymes in cell lysates or tissue homogenates are in a single PDF file here.
Glutathione Enzymatic Recycling Assay
This interactive Excel workbook guides the user through the protocol to measure total glutathione and disulfide gluthione levels in cell lysates or tissue homogenates using the enzymatic recycling method. Currently available only in Brazilian Portuguese here.
Chemical In Vitro "Antioxidant"* Assays
ABTS decolorization assay – in vitro antioxidant capacity. Formally cited in these publications.
DPPH decolorization assay - in vitro antioxidant capacity
*Note that these radical scavenging assays have many limitations and are not very good predictors of actual antioxidant effects in living organisms. See: 10.1016/j.freeradbiomed.2013.05.045 and 10.1089/ars.2015.6393
Protein concentration quantitation
Bradford protein assay – Protein concentration measurement (single 595 nm read)
Bradford protein assay – Protein concentration measurement (A590/A450 improved linearity)
Bradford protein assay – Protein concentration measurement using RGB data
Oxidative Stress Markers Assays
🚧🚧🚧 Section under construction.
Other Assays
🚧🚧🚧 Section under construction.